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Gradient PCR and Q-PCR (molecular biology platform)

Gradient PCR and Q-PCR (molecular biology platform)

Cégep de Shawinigan (CNETE)

Place :

Shawinigan

Infrastructure type :

Equipment

/// Features:
- Block and lids with a capacity for very rapid heating and cooling (PCR and qPCR)
- Independent gradient thermal function programming spread over 12 columns (PCR and qPCR)
- Gradient range of over 20 °C (PCR and qPCR), from 4 to 99 °C
- Vapo.protect technology reducing evaporation from the wells (qPCR)
- LED lights emit blue light at 470 nm and excite fluorophores
- (SYBR®Green, FAM, VIC, TET, HEX, ROX, JOE, TAMRA) (qPCR)
- Possibility to choose primers and probes such as Taqman, Beacon, and SYBR (qPCR)
- Continuous monitoring of DNA amplification and post-amplification analysis software

/// Examples of applications:
- Amplification of target genes in various microorganisms (PCR)
- Detection and quantification of target genes (ex. proteins, enzyme) of a variety of microorganisms (qPCR)
- Monitoring bacterial population in bioprocesses (qPCR)
- Monitoring of gene expression using ARNm and genotype variation
- Optimization of process conditions through pathogen detection (presence/absence)

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